The Franciscan monastery and the church of St. Anthony of Padua in Lychakiv (49 Lychakivska Street) were founded in 1718 by the Kraków castellan Janusz (in another version - Januarius) Antoni Wyszniewiecki, and in 1739 it was consecrated by the Lviv suffragan bishop Samuel Glowinski. The original wooden buildings of the Franciscans were located on the same site a hundred years earlier (1617).

After the Austrian authorities abolished a significant number of Catholic orders, the Franciscan monastery was also closed (1784), and the church of St. Anthony became a parish church. In the middle of the 18th century, a sculptural figure of St. Anthony of Padua was installed near the church, and on the front steps - a sculpture of the Immaculate Conception of the Virgin Mary and angels (the statues of St. Anthony and the Virgin Mary belong to the sculptor sculptor Sebastian Fessinger). In 1818, according to the plans of architect Josef Markl, a bell tower was built, and a few years later the church was surrounded by a wall (1830). In 1900, some reconstructions were carried out and the stairs were restored, after which the sculptures of angels on both sides of the stairs were removed. For the needs of the army during World War I, as in all Lviv churches, the church bells were confiscated.

After the war, the Church of St. Anthony, like the Latin Cathedral, was open to the faithful, but a music school was located in the presbytery. With the independence of Ukraine, the church was returned to the Franciscans.